Clean and disinfect your work area. Put on gloves and ensure all supplies are within reach. Remove peptide vial from refrigerator and allow to reach room temperature (about 10-15 minutes).
Determine how much bacteriostatic water to add based on your desired concentration. Common ratios:
If you have 5mg of peptide and want 1mg/mL concentration:
5mg ÷ 1mg/mL = 5mL of bacteriostatic water needed
Final result: 5mL solution at 1mg/mL concentration
Attach needle to syringe. Draw the calculated amount of bacteriostatic water into the syringe. Remove any air bubbles by gently tapping the syringe and pushing the plunger slightly.
Wipe the rubber stopper of the peptide vial with an alcohol swab. Allow to air dry for 10-15 seconds.
CRITICAL: Insert needle into vial at a 45-degree angle. Slowly inject water down the side of the vial, NOT directly onto the powder. This prevents foaming and protein degradation.
DO NOT shake the vial. Instead, gently swirl or roll the vial between your palms. The peptide should dissolve completely within 1-2 minutes. If cloudiness persists, continue gentle swirling.
The final solution should be clear and colorless. If you see:
Label the vial with:
Store in refrigerator immediately at 2-8°C.
Store lyophilized peptides in freezer (-20°C) or refrigerator (2-8°C) depending on manufacturer instructions. Protect from light and moisture.
Store in refrigerator at 2-8°C. Most peptides remain stable for 14-30 days when properly stored. Never freeze reconstituted peptides.
Discard reconstituted peptide if you notice:
Try gently warming the vial in your hands or adding slightly more bacteriostatic water. Some peptides may take longer to dissolve.
Continue gentle swirling. If cloudiness persists after 5 minutes, the peptide may be degraded or contaminated.
Indicates too rapid injection. Let foam settle and avoid agitation. For future reconstitutions, inject more slowly.